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Targeted proteomics of signal pathways in BV2 microglial cell activation

ABSTRACT: Microglia become activated by disturbances in the homeostasis of their local microenvironment. While there are varying degrees of microglia activation, a pro-inflammatory reactive state induced by exposure to stimuli like LPS and IFN-γ. In this study, using label-free PRM approach which is allowing us to measure the hyper-multiplex quantitative value of each protein with high-resolution mass spectrometry, we have quantified over 450 peptides at single run corresponding with the proteins belong to the pathways and directly or indirectly interacted proteins based on STRING database.Moreover, we performed longitudinal quantification of secreted proteins during the detrimental activation of microglia which releases neurotoxic molecules mediated neuronal cell loss in the brain region.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Cell Culture, Macrophage

DISEASE(S): Alzheimer's Disease

SUBMITTER: Jongmin Woo

LAB HEAD: Youngsoo Kim

PROVIDER: PXD006556 | Pride | 2017-08-09

REPOSITORIES: Pride

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Quantitative Proteomics Reveals Temporal Proteomic Changes in Signaling Pathways during BV2 Mouse Microglial Cell Activation.

Woo Jongmin J Han Dohyun D Wang Joseph Injae JI Park Joonho J Kim Hyunsoo H Kim Youngsoo Y

Journal of proteome research 20170818 9

The development of systematic proteomic quantification techniques in systems biology research has enabled one to perform an in-depth analysis of cellular systems. We have developed a systematic proteomic approach that encompasses the spectrum from global to targeted analysis on a single platform. We have applied this technique to an activated microglia cell system to examine changes in the intracellular and extracellular proteomes. Microglia become activated when their homeostatic microenvironme ...[more]

PMID: 28777000

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