Dataset Information

Construction of functional tissue-engineered microvasculatures by using circulating fibrocytes as mural cells

ABSTRACT: Mural cells are essential to the proper function of microvasculatures. However, the feasible cell source for mural cells has not been found, which is one of the key obstacles facedin tissue engineering. Our data show that circulating fibrocytes (CFs) sheathed and stabilized the microvasculatures formed by vascular endothelial cells (VECs) in collagen gel, formed gap junctions with VECs, and induced formation of basement membrane. When transplanted into nude mice with VECs either in collagen gel or Matrigel, CFs sheathed the microvasculatures formed by VECs, induced basal membrane formation and the microvasculatures connected to the host circulation. Human brain pericytes (HBPCs) had similar function to CFs, but HBPCs often mosaic into the lumen of capillary-like structures, actively proliferate, and have lower capacity of endocytosis and migration. Based on these data, we concluded thatCFs can be used as the cell source for mural cells to generate tissue-engineered microvasculatures

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Cell Culture, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Jinqing Li

LAB HEAD: Jinqing Li

PROVIDER: PXD006763 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CF-VEC_Pericyte-VEC_proteinGroups.txt	Txt
	CF-VEC_Pericyte_proteinGroups_PROCESSED.xlsx	Xlsx
	CF-VEC_Pericytes_proteinGroups.txt	Txt
	CF_1a.msf	Msf
	CF_1a.raw	Raw

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Publications

Construction of functional tissue-engineered microvasculatures using circulating fibrocytes as mural cells.

Wang Xiaolin X Tan Hong H Gunawardena Harsha P HP Cao Jin J Dang Lu L Deng Hongbing H Li Xueyong X Wang Hongjun H Li Jinqing J Cui Caibin C Gerber David A DA

Journal of tissue engineering 20250128

Mural cells are essential for maintaining the proper functions of microvasculatures. However, a key challenge of microvascular tissue engineering is identifying a cellular source for mural cells. We showed that <i>in vitro</i>, circulating fibrocytes (CFs) can (1) shear and stabilize the microvasculatures formed by vascular endothelial cells (VECs) in a collagen gel, (2) form gap junctions with VECs and (3) induce basement membrane formation. CFs transplanted into nude mice along with VECs in ei ...[more]

PMID: 39882546

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