Dataset Information


Mass spectrometry analysis of proteins interacting with STAT3

ABSTRACT: Mass spectrometry analysis of proteins interacting with STAT3¬Flag was performed. STAT3-Flag was transiently expressed in HeLa cells and the interactome of STAT3-Flag was studied in lysosomal extracts. proteins co-¬immunoprecipitating with STAT3--Flag identified eight subunits of the vacuolar H+-¬ ATPase (V-¬ATPase) as potential STAT3 binding partners. Validation experiments were performed by antibody pull-down of STAT3.


ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Hela Cell

SUBMITTER: Johan Palmfeldt  

LAB HEAD: Johan Palmfeldt

PROVIDER: PXD006788 | Pride | 2018-08-29


Dataset's files

Action DRS
228_Bin-pCDH-flag1.raw Raw
229_Bin-hStat-Flag1.raw Raw
230_Bin-hStat3-NCI.raw Raw
2909-Bin-cross-1A.raw Raw
2911-Bin-cross-2A.raw Raw
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STAT3 associates with vacuolar H<sup>+</sup>-ATPase and regulates cytosolic and lysosomal pH.

Liu Bin B   Palmfeldt Johan J   Lin Lin L   Colaço Alexandria A   Clemmensen Knut K B KKB   Huang Jinrong J   Xu Fengping F   Liu Xin X   Maeda Kenji K   Luo Yonglun Y   Jäättelä Marja M  

Cell research 20180820 10

Dysregulated intracellular pH is emerging as a hallmark of cancer. In spite of their acidic environment and increased acid production, cancer cells maintain alkaline intracellular pH that promotes cancer progression by inhibiting apoptosis and increasing glycolysis, cell growth, migration, and invasion. Here we identify signal transducer and activator of transcription-3 (STAT3) as a key factor in the preservation of alkaline cytosol. STAT3 associates with the vacuolar H<sup>+</sup>-ATPase in a c  ...[more]

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