Proteomics

Dataset Information

35

Mitotic post-translational modifications of histones promote chromatin compaction in vitro


ABSTRACT: As histones undergo major changes in their post-translational modifications during mitotic entry, we speculated that the spectrum of cell cycle-specific histone modifications might contribute to chromosome compaction during mitosis. To test this hypothesis, we isolated core histones from interphase and mitotic cells and reconstituted chromatin with them. We used mass spectrometry to show that key post-translational modifications remained intact during our isolation procedure.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Gallus gallus  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Juan Jose Bonfiglio  

LAB HEAD: William Earnshaw

PROVIDER: PXD007116 | Pride | 2018-10-24

REPOSITORIES: Pride

Dataset's files

Source:
altmetric image

Publications

Mitotic post-translational modifications of histones promote chromatin compaction <i>in vitro</i>.

Zhiteneva Alisa A   Bonfiglio Juan Jose JJ   Makarov Alexandr A   Colby Thomas T   Vagnarelli Paola P   Schirmer Eric C EC   Matic Ivan I   Earnshaw William C WC  

Open biology 20170901 9


How eukaryotic chromosomes are compacted during mitosis has been a leading question in cell biology since the nineteenth century. Non-histone proteins such as condensin complexes contribute to chromosome shaping, but appear not to be necessary for mitotic chromatin compaction. Histone modifications are known to affect chromatin structure. As histones undergo major changes in their post-translational modifications during mitotic entry, we speculated that the spectrum of cell-cycle-specific histon  ...[more]

Similar Datasets

2017-01-01 | S-EPMC5627050 | BioStudies
2008-01-01 | S-EPMC2586264 | BioStudies
2019-01-01 | S-EPMC6589789 | BioStudies
1000-01-01 | S-EPMC4378191 | BioStudies
2012-01-01 | S-EPMC3374815 | BioStudies
2012-01-01 | S-EPMC3535264 | BioStudies
1974-01-01 | S-EPMC1166252 | BioStudies
2016-01-01 | S-EPMC4946945 | BioStudies
1000-01-01 | S-EPMC3784377 | BioStudies
2011-01-01 | S-EPMC3193423 | BioStudies