EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

ABSTRACT: We developed EASI-tag (Easily Abstractable Sulfoxide-based Isobaric tag), a new generation of amine-derivatizing and sulfoxide-containing isobaric labelling reagents, which dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level enable accurate determination of quantitative differences between multiplexed samples. EASI-tag makes isobaric labeling applicable to any bottom up proteomics application and to benchtop mass spectrometers.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Epithelial Cell, Hela Cell

SUBMITTER: Sebastian Virreira Winter  

LAB HEAD: Matthias Mann

PROVIDER: PXD007165 | Pride | 2018-06-21

REPOSITORIES: Pride