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Proteomic analysis of the apoplast reveals a differential cold acclimation response among winter wheat crown tissues

ABSTRACT: The crown is the critical region for survival of winter wheat exposed to low temperature stresses. When wheat is exposed to non-freezing low temperatures, they can increase their freezing tolerance (cold acclimation, ACC). Changes within the apoplast are thought to be crucial for acquisition of freezing tolerance. However, how individual tissues within the ccrown, namely the shoot apical meristem (SAM, responsible for new shoot growth) and vascular transition zone (VTZ, located at the base of the crown)enhance tolerance to freezing has not yet been characterized. In the present study, we conducted shotgun proteomic analysis of the apoplast fluid to investigate ACC-induced proteins in the SAM and VTZ.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Viridiplantae

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Ian Willick

LAB HEAD: Matsuo Uemura

PROVIDER: PXD007796 | Pride | 2018-10-23

REPOSITORIES: Pride

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Tissue-specific changes in apoplastic proteins and cell wall structure during cold acclimation of winter wheat crowns.

Willick Ian R IR Takahashi Daisuke D Fowler D Brian DB Uemura Matsuo M Tanino Karen K KK

Journal of experimental botany 20180201 5

The wheat (Triticum aestivum L.) crown is the critical organ of low temperature stress survival over winter. In cold-acclimated crowns, ice formation in the apoplast causes severe tissue disruption as it grows at the expense of intracellular water. While previous crown studies have shown the vascular transition zone (VTZ) to have a higher freezing sensitivity than the shoot apical meristem (SAM), the mechanism behind the differential freezing response is not fully understood. Cooling cold-acclim ...[more]

PMID: 29373702

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