Proteomic analysis of Fusobacterium nucleatum and Porphyromonas gingivalis biofilms and planktonic cultures when grown as mono- or dual-species models
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ABSTRACT: Bacterial cells in biofilms adopt a profoundly different phenotype than their planktonic counterparts. The Gram-negative bacteria Fusobacterium nucleatum and Porphyromonas gingivalis are pathogens associated with oral biofilm and periodontal disease. In this study, we identified and functionally characterized the proteome of F. nucleatum and P. gingivalis cells grown either as biofilms or in planktonic cultures by high-resolution liquid chromatography-tandem mass spectrometry. We used proteomic analysis for identification and label-free quantification of proteins, and sequence-based functional characterization for their classification. In F. nucleatum biofilm five proteins showed changes from planktonic condition, including proteins involved in vitamin B metabolic processes. Forty proteins were changed in P. gingivalis biofilm, 30 increased and 10 decreased. Putative cell division trigger factor and riboflavin biosynthesis proteins were the most increased in this biofilm. To describe interactions between the two species at the protein level, we grew the bacteria either individually or together. In the mixed species biofilm culture, 112 proteins showed significant changes, with 72 proteins derived from F. nucleatum and 40 proteins from P. gingivalis. By comparing dual-species to mono-species in biofilm and in planktonic growth conditions, P. gingivalis showed more proteins with decreased level in the dual species conditions.
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Fusobacterium Nucleatum Subsp. Nucleatum Atcc 25586 Porphyromonas Gingivalis Atcc 33277
SUBMITTER: Marwan Mansoor Ali Mohammed
LAB HEAD: Vidar Bakken
PROVIDER: PXD008288 | Pride | 2021-09-22
REPOSITORIES: Pride
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