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Aspartyl Protease 5 matures virulence factors found at the host-parasite interface in Toxoplasma gondii

ABSTRACT: N-terminome analysis of WT and ASP5 KO Toxoplasma parasites.

INSTRUMENT(S): Bruker Daltonics instrument model, Q Exactive

ORGANISM(S): Toxoplasma Gondii Me49 Toxoplasma Gondii

TISSUE(S): Cell Suspension Culture

SUBMITTER: Laura Dagley

LAB HEAD: Christopher J Tonkin

PROVIDER: PXD008574 | Pride | 2018-10-15

REPOSITORIES: Pride

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Aspartyl Protease 5 Matures Dense Granule Proteins That Reside at the Host-Parasite Interface in Toxoplasma gondii.

Coffey Michael J MJ Dagley Laura F LF Seizova Simona S Kapp Eugene A EA Infusini Giuseppe G Roos David S DS Boddey Justin A JA Webb Andrew I AI Tonkin Christopher J CJ

mBio 20181030 5

Toxoplasma gondii infects approximately 30% of the world's population, causing disease primarily during pregnancy and in individuals with weakened immune systems. Toxoplasma secretes and exports effector proteins that modulate the host during infection, and several of these proteins are processed by the Golgi-associated aspartyl protease 5 (ASP5). Here, we identify ASP5 substrates by selectively enriching N-terminally derived peptides from wild-type and Δasp5 parasites. We r ...[more]

PMID: 30377279

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