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Identification of adsorbing human plasma proteins on alginate gel beads

ABSTRACT: The biocompatibility of immunoisolation devices is a major challenge for their use in cellular therapies. Utilisation of alginate for the encapsulation of pancreatic islets as a potential cellular therapy for type 1 diabetes has shown to be limited in terms of long-term graft survival, due to pericapsular fibrotic overgrowth. Fibrotic overgrowth is a complex process that involves several factors, including protein adsorption. This work investigates the protein adsorption profiles of plasma incubated alginate beads for the identification of key proteins that may play a role in the biocompatibility of this biomaterial.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood Plasma

SUBMITTER: Animesh Sharma

LAB HEAD: Geir Slupphaug

PROVIDER: PXD009135 | Pride | 2023-03-10

REPOSITORIES: Pride

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MS-proteomics provides insight into the host responses towards alginate microspheres.

Coron Abba E AE Fonseca Davi M DM Sharma Animesh A Slupphaug Geir G Strand Berit L BL Rokstad Anne Mari A AMA

Materials today. Bio 20221111

Protein adsorption to biomaterial surfaces is considered a determining factor for the host response. Here we detail the protein adsorption profiles of alginate hydrogel microspheres relevant for cell therapy using mass spectrometry (MS)-based proteomics. The investigated microspheres include sulfated alginate (SA), high G alginate (HiG), and poly-l-lysine coated alginate (AP), which previously have been shown to exhibit different inflammatory and fibrotic responses. The biological significance w ...[more]

PMID: 36420052

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