Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Suspension Culture, Cell Culture, Erythroblast
SUBMITTER: Joseph Hawksworth
LAB HEAD: Ashley Mark Toye
PROVIDER: PXD009291 | Pride | 2018-04-26
REPOSITORIES: Pride
EMBO molecular medicine 20180601 6
Regular blood transfusion is the cornerstone of care for patients with red blood cell (RBC) disorders such as thalassaemia or sickle-cell disease. With repeated transfusion, alloimmunisation often occurs due to incompatibility at the level of minor blood group antigens. We use CRISPR-mediated genome editing of an immortalised human erythroblast cell line (BEL-A) to generate multiple enucleation competent cell lines deficient in individual blood groups. Edits are combined to generate a single cel ...[more]