Dataset Information

Barley seed proteome analysis - Genes for seed longevity in barley identified by genomic analysis on near isogenic lines

ABSTRACT: Genes controlling differences in seed longevity between two barley (Hordeum vulgare) accessions were identified by combining a quantitative genetics approach with ˈomicsˈ technologies in Near Isogenic Lines (NILs). The NILs were derived from crosses between the spring barley landraces L94 from Ethiopia and Cebada Capa from Argentina, which produce short-lived and long-lived seeds, respectively. The NILs harbor introgressions from Cebada Capa in four QTLs for seed longevity on 1H and 2H in the background of L94. A label-free proteome analysis was performed on mature, non aged seeds of the two parental lines and the L94 NILs.

INSTRUMENT(S):

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Plant Cell, Seed

SUBMITTER: Iris Finkemeier

LAB HEAD: Prof. Iris Finkemeier

PROVIDER: PXD009464 | Pride | 2018-07-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	151207_MK_DoWo_FLTQ_10_sdb1.raw	Raw
	151207_MK_DoWo_FLTQ_10_sdb2.raw	Raw
	151207_MK_DoWo_FLTQ_10_sdb3.raw	Raw
	151207_MK_DoWo_FLTQ_11_sdb1.raw	Raw
	151207_MK_DoWo_FLTQ_11_sdb2.raw	Raw

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Publications

Genes for seed longevity in barley identified by genomic analysis on near isogenic lines.

Wozny Dorothee D Kramer Katharina K Finkemeier Iris I Acosta Ivan F IF Koornneef Maarten M

Plant, cell & environment 20180619 8

Genes controlling differences in seed longevity between 2 barley (Hordeum vulgare) accessions were identified by combining quantitative genetics "omics" technologies in near isogenic lines (NILs). The NILs were derived from crosses between the spring barley landraces L94 from Ethiopia and Cebada Capa from Argentina. A combined transcriptome and proteome analysis on mature, nonaged seeds of the 2 parental lines and the L94 NILs by RNA-sequencing and total seed proteomic profiling identified the U ...[more]

PMID: 29744896

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