Profiling the proteomic inflammatory state of human astrocytes using DIA mass spectrometry

ABSTRACT: In the present study we used DIA MS to characterize the profile of over 4,000 proteins in immortalized human astrocytes exposed to TNF, IL-1β, and LPS, as well as in primary human astrocytes cocultured with brain endothelial cells and exposed to LPS. For both immortalized and primary astrocytes, DIA MS data were matched against a spectral library generated by merging DDA MS runs from both unfractionated and fractionated cell lysates.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Astrocyte

SUBMITTER: Vito Dozio  

LAB HEAD: Jean-Charles Sanchez

PROVIDER: PXD009891 | Pride | 2018-12-10

REPOSITORIES: Pride