Proteomics

Dataset Information

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Profiling the proteomic inflammatory state of human astrocytes using DIA mass spectrometry


ABSTRACT: In the present study we used DIA MS to characterize the profile of over 4,000 proteins in immortalized human astrocytes exposed to TNF, IL-1β, and LPS, as well as in primary human astrocytes cocultured with brain endothelial cells and exposed to LPS. For both immortalized and primary astrocytes, DIA MS data were matched against a spectral library generated by merging DDA MS runs from both unfractionated and fractionated cell lysates.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Astrocyte

SUBMITTER: Vito Dozio  

LAB HEAD: Jean-Charles Sanchez

PROVIDER: PXD009891 | Pride | 2018-12-10

REPOSITORIES: Pride

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Publications

Profiling the proteomic inflammatory state of human astrocytes using DIA mass spectrometry.

Dozio Vito V   Sanchez Jean-Charles JC  

Journal of neuroinflammation 20181130 1


<h4>Background</h4>Astrocytes are the most abundant cells in the central nervous system and are responsible for a wide range of functions critical to normal neuronal development, synapse formation, blood-brain barrier regulation, and brain homeostasis. They are also actively involved in initiating and perpetuating neuroinflammatory responses. However, information about their proteomic phenotypes under inflammation is currently limited.<h4>Method</h4>Data-independent acquisition mass spectrometry  ...[more]

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