Proteomics

Dataset Information

25

Smell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins


ABSTRACT: Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on host fish are likely to cause release of similar alarm cues into the environment due to the stress, wounding and immune response stimulated upon infection. Alarm cues are often released in the form of proteins and peptides and provide important insights into bodily function and infection status. Here we outline a non-invasive method to identify potential chemical cues associated with infection in fish by extracting, purifying and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirmed that proteins could be characterized from teleost water samples and that variation in protein profiles could be detected between infected and uninfected individuals and fish and parasite only water samples. Our novel assay provides a non-invasive method for assessing the health condition of both wild and farmed aquatic organisms. Similar to environmental DNA monitoring methods, these proteomic techniques could provide an important tool in applied biology and aquaculture biology

INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS

ORGANISM(S): Kryptolebias marmoratus  

TISSUE(S): Other Source

DISEASE(S): Not Available

SUBMITTER: Rebecca Pawluk  

LAB HEAD: Prof Peter M Brophy

PROVIDER: PXD010987 | Pride | 2019-01-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
171006-Blank1.mzML Mzml
171006-Blank2.mzML Mzml
171006-Blank3.mzML Mzml
171006-Blank4.mzML Mzml
171006-Sample2.RAW Raw
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Publications

Smell of Infection: A Novel, Noninvasive Method for Detection of Fish Excretory-Secretory Proteins.

Pawluk Rebecca J RJ   Stuart Rebekah R   Garcia de Leaniz Carlos C   Cable Joanne J   Morphew Russell M RM   Brophy Peter M PM   Consuegra Sofia S  

Journal of proteome research 20190109 3


Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to the stress, wounding, and immune response stimulated upon infection. Alarm cues are often released in the form of proteins, antimicrobial peptides, and immunoglobulins that provide important insights into bodily function and infection status. Here we outline a  ...[more]

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