Proteomics,Multiomics

Dataset Information

0

TranslationalLandscape_HumanHeart

ABSTRACT: Here, we analyze the expression of predicted microproteins in human heart tissue and iPSC cardiomyocytes, using shot-gun proteomics and SRM. 4 identified candidates were functionally validated by interactome studies using AP-MS (affinity purification coupled to mass spectrometry). In addition, the impact of disease-causing protein-truncating variants on translation was studied in rat heart tissue by performing shot-gun proteomics.

OTHER RELATED OMICS DATASETS IN: EGAS00001003263EGAC00001001040EGAD00001004394EGAO00000000992MODEL1109130000MODEL1311110000MODEL1909260003MODEL1311110001MODEL1909260004MODEL1703310000MODEL1909260005MODEL1909260006

INSTRUMENT(S): TSQ Quantiva, Orbitrap Fusion, Q Exactive

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human)

TISSUE(S): Heart, Cell Culture

SUBMITTER: Marieluise Kirchner  

LAB HEAD: Norbert Hubner

PROVIDER: PXD012593 | Pride | 2019-06-04

REPOSITORIES: Pride

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Publications

The Translational Landscape of the Human Heart.

van Heesch Sebastiaan S   Witte Franziska F   Schneider-Lunitz Valentin V   Schulz Jana F JF   Adami Eleonora E   Faber Allison B AB   Kirchner Marieluise M   Maatz Henrike H   Blachut Susanne S   Sandmann Clara-Louisa CL   Kanda Masatoshi M   Worth Catherine L CL   Schafer Sebastian S   Calviello Lorenzo L   Merriott Rhys R   Patone Giannino G   Hummel Oliver O   Wyler Emanuel E   Obermayer Benedikt B   Mücke Michael B MB   Lindberg Eric L EL   Trnka Franziska F   Memczak Sebastian S   Schilling Marcel M   Felkin Leanne E LE   Barton Paul J R PJR   Quaife Nicholas M NM   Vanezis Konstantinos K   Diecke Sebastian S   Mukai Masaya M   Mah Nancy N   Oh Su-Jun SJ   Kurtz Andreas A   Schramm Christoph C   Schwinge Dorothee D   Sebode Marcial M   Harakalova Magdalena M   Asselbergs Folkert W FW   Vink Aryan A   de Weger Roel A RA   Viswanathan Sivakumar S   Widjaja Anissa A AA   Gärtner-Rommel Anna A   Milting Hendrik H   Dos Remedios Cris C   Knosalla Christoph C   Mertins Philipp P   Landthaler Markus M   Vingron Martin M   Linke Wolfgang A WA   Seidman Jonathan G JG   Seidman Christine E CE   Rajewsky Nikolaus N   Ohler Uwe U   Cook Stuart A SA   Hubner Norbert N  

Cell 20190530 1

Gene expression in human tissue has primarily been studied on the transcriptional level, largely neglecting translational regulation. Here, we analyze the translatomes of 80 human hearts to identify new translation events and quantify the effect of translational regulation. We show extensive translational control of cardiac gene expression, which is orchestrated in a process-specific manner. Translation downstream of predicted disease-causing protein-truncating variants appears to be frequent, s  ...[more]

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