Proteomics

Dataset Information

0

Carboxylic acid consumption and production by Corynebacterium glutamicum_LC-MS/MS


ABSTRACT: Corynebacterium glutamicum is well-known as an industrial workhorse, most notably for its use in the bulk production of amino acids in the feed and food sector. Fast growth and robustness against oscillatory oxygen availability, which can occur in large-scale bioreactors, are advantageous properties of this bacterium. However, previous studies of the effect of gradients in scale-down reactors with complex media disclosed an accumulation of several carboxylic acids and a parallel decrease of growth and product accumulation by C. glutamicum. This study addresses the impact of carboxylic acids, e.g. acetate and L-lactate, on the cultivation process and their potential role in scale up related performance losses. In order to mimic a discontinuous oxygen supply, a fluctuating power input in shake flask and stirred tank cultivations with mineral salt was applied. One focus of this study is to identify relative changes in the proteome due to the differing availability of carboxylic acids under discontinuous oxygen supply.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Corynebacterium glutamicum ATCC 13032  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Bianca Klein  

LAB HEAD: Marco Oldiges

PROVIDER: PXD012760 | Pride | 2019-03-14

REPOSITORIES: pride

altmetric image

Publications

Carboxylic acid consumption and production by Corynebacterium glutamicum.

Conrady Marius M   Lemoine Anja A   Limberg Michael H MH   Oldiges Marco M   Neubauer Peter P   Junne Stefan S  

Biotechnology progress 20190323 3


Corynebacterium glutamicum is well-known as an industrial workhorse, most notably for its use in the bulk production of amino acids in the feed and food sector. Previous studies of the effect of gradients in scale-down reactors with complex media disclosed an accumulation of several carboxylic acids and a parallel decrease of growth and product accumulation. This study, therefore, addresses the impact of carboxylic acids, for example, acetate and l-lactate, on the cultivation of the cadaverine p  ...[more]

Similar Datasets

2019-08-12 | PXD013751 | Pride
2014-03-26 | E-GEOD-56119 | ArrayExpress
2007-10-16 | GSE8035 | GEO
2007-10-16 | GSE8089 | GEO
2007-10-16 | GSE8088 | GEO
2008-06-15 | E-GEOD-8035 | ArrayExpress
2008-06-15 | E-GEOD-8088 | ArrayExpress
2008-06-15 | E-GEOD-8089 | ArrayExpress
2018-10-01 | PXD009088 | Pride
| GSE99546 | GEO