A closer look at Aspergillus: Online monitoring via scattered light enables reproducible phenotyping

ABSTRACT: Development of microtiter plate based microbioreactor cultivation for Aspergillus giganteus with quasi-continuous online measurements. Different parameters such as well geometry, shaking frequency and morphology controlling agents were investigated in order to optimize the microtiter plate cultivation and scattered light signal towards reproducibility and homogeneity. An optimized medium was developed and scalability into stirred tank bioreactor cultivation was analyzed. As a transferability indicator the supernatant of both cultivation systems was analyzed for secreted protein patterns with a focus on an antifungal protein (AFP) and alpha-sarcin. These proteins were identified via LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Aspergillus Giganteus

SUBMITTER: Bianca Klein  

LAB HEAD: Marco Oldiges

PROVIDER: PXD013751 | Pride | 2019-08-12

REPOSITORIES: Pride