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Quantitative phosphoproteome analyses of human macrophage-like THP-1 cells following infection with virulent Mycobacterium tuberculosis and avirulent M. bovis BCG, respectively.

ABSTRACT: In this project we conducted a comprehensive analysis of expression and global phosphorylation status of the host proteins following infection with virulent and avirulent mycobacteria. Our objective is to identify previously uncharacterized proteins of host macrophages that are specifically expressed and phosphorylated in response to Mtb and BCG, respectively and may play important role in regulating their intracellular survival

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Macrophage

DISEASE(S): Pulmonary Tuberculosis

SUBMITTER: Renu Goel

LAB HEAD: Dr. Nisheeth Agarwal

PROVIDER: PXD013171 | Pride | 2020-05-15

REPOSITORIES: Pride

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Relative and Quantitative Phosphoproteome Analysis of Macrophages in Response to Infection by Virulent and Avirulent Mycobacteria Reveals a Distinct Role of the Cytosolic RNA Sensor RIG-I in Mycobacterium tuberculosis Pathogenesis.

Choudhary Eira E Bullen C Korin CK Goel Renu R Singh Alok Kumar AK Praharaj Monali M Thakur Preeti P Dhiman Rohan R Bishai William R WR Agarwal Nisheeth N

Journal of proteome research 20200514 6

Comparative phosphoproteomics of Mycobacterium tuberculosis (Mtb)- and Mycobacterium bovis BCG (BCG)-infected macrophages could be instrumental in understanding the characteristic post-translational modifications of host proteins and their subsequent involvement in determining Mtb pathogenesis. To identify proteins acquiring a distinct phosphorylation status, herein, we compared the phosphorylation profile of macrophages upon exposure to Mtb and BCG. We observed a significant depho ...[more]

PMID: 32407090

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