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The metabolic response of Pseudomonas taiwanensis to NADH dehydrogenase deficiency

ABSTRACT: We investigated the essentiality of the three NADH dehydrogenases of the respiratory chain of the obligate aerobe Pseudomonas taiwanensis VLB120 and the impact of the knockouts of corresponding genes on its physiology and metabolism.

INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS

ORGANISM(S): Pseudomonas Taiwanensis

TISSUE(S): Cell Culture

SUBMITTER: Christopher Petzold

LAB HEAD: Christopher Petzold

PROVIDER: PXD013623 | Pride | 2020-04-08

REPOSITORIES: Pride

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Systems Analysis of NADH Dehydrogenase Mutants Reveals Flexibility and Limits of Pseudomonas taiwanensis VLB120's Metabolism.

Nies Salome C SC Dinger Robert R Chen Yan Y Wordofa Gossa G GG Kristensen Mette M Schneider Konstantin K Büchs Jochen J Petzold Christopher J CJ Keasling Jay D JD Blank Lars M LM Ebert Birgitta E BE

Applied and environmental microbiology 20200519 11

Obligate aerobic organisms rely on a functional electron transport chain for energy conservation and NADH oxidation. Because of this essential requirement, the genes of this pathway are likely constitutively and highly expressed to avoid a cofactor imbalance and energy shortage under fluctuating environmental conditions. We here investigated the essentiality of the three NADH dehydrogenases of the respiratory chain of the obligate aerobe Pseudomonas taiwanensis VLB120 and the impact of th ...[more]

PMID: 32245760

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