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Proteomic analysis of Angomonas deanei

ABSTRACT: The transformation of endosymbiotic bacteria into genetically integrated organelles was central to eukaryote evolution. During organellogenesis, control over endosymbiont division, proteome composition, and physiology largely shifted from endosymbiont to nucleus. However, the order and timing of events underpinning this major evolutionary transition are poorly understood. Here, we identified by protein mass spectrometry seven nucleus-encoded proteins that are targeted to the endosymbiont.

INSTRUMENT(S): Q Exactive Plus, LTQ Orbitrap Elite

ORGANISM(S): Angomonas Deanei Candidatus Kinetoplastibacterium Crithidii (ex Angomonas Deanei Atcc 30255)

TISSUE(S): Cell Culture

SUBMITTER: Gereon Poschmann

LAB HEAD: Gereon Poschmann

PROVIDER: PXD017908 | Pride | 2023-04-25

REPOSITORIES: Pride

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Host-symbiont interactions in Angomonas deanei include the evolution of a host-derived dynamin ring around the endosymbiont division site.

Morales Jorge J Ehret Georg G Poschmann Gereon G Reinicke Tobias T Maurya Anay K AK Kröninger Lena L Zanini Davide D Wolters Rebecca R Kalyanaraman Dhevi D Krakovka Michael M Bäumers Miriam M Stühler Kai K Nowack Eva C M ECM

Current biology : CB 20221207 1

The trypanosomatid Angomonas deanei is a model to study endosymbiosis. Each cell contains a single β-proteobacterial endosymbiont that divides at a defined point in the host cell cycle and contributes essential metabolites to the host metabolism. Additionally, one endosymbiont gene, encoding an ornithine cyclodeaminase (OCD), was transferred by endosymbiotic gene transfer (EGT) to the nucleus. However, the molecular mechanisms mediating the intricate host/symbiont interactions are largely unexpl ...[more]

PMID: 36480982

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