Tandem affinity purification of monobodies against STAT3

ABSTRACT: The aim of the proteomics experiment was to determine the specificity of the generated monobodies for the target protein STAT3. This was done in an affinity-enrichment experiment using two endogenously expressed and tandem-affinity-tagged monobodies in two different cell lines (Jurkat and K562). Eluted proteins after affinity purification were resolved by SDS-PAGE, and analysed using bottom-up proteomics workflow including tryptic digestion, LC-MS/MS, database-search and spectral counting.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Leukocyte, Bone Marrow, Blood

DISEASE(S): Acute Leukemia,Chronic Myeloid Leukemia

SUBMITTER: Grégory La Sala  

LAB HEAD: Oliver Hantschel

PROVIDER: PXD018374 | Pride | 2020-07-10

REPOSITORIES: Pride