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Analysis of protein expression change upon RBMS1 knockdown

ABSTRACT: To assess how dpeletion of RBMS1 affects the proteome, SW480 colon adenocarcinoma cells expressing control or RBMS1 shRNAs were compared by Tandem Mass Tagging (TMT) protoemics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Faraz Mardakheh

LAB HEAD: Faraz Mardakheh

PROVIDER: PXD019478 | Pride | 2020-06-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20200306_set3_SW480celldensity1_A_R1.raw	Raw
	20200306_set3_SW480celldensity1_A_R2.raw	Raw
	20200306_set3_SW480celldensity1_B_R1.raw	Raw
	20200306_set3_SW480celldensity1_B_R2.raw	Raw
	20200306_set3_SW480celldensity1_C_R1.raw	Raw

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Publications

RBMS1 Suppresses Colon Cancer Metastasis through Targeted Stabilization of Its mRNA Regulon.

Yu Johnny J Navickas Albertas A Asgharian Hosseinali H Culbertson Bruce B Fish Lisa L Garcia Kristle K Olegario John Paolo JP Dermit Maria M Dodel Martin M Hänisch Benjamin B Luo Yikai Y Weinberg Ethan M EM Dienstmann Rodrigo R Warren Robert S RS Mardakheh Faraz K FK Goodarzi Hani H

Cancer discovery 20200608 9

Identifying master regulators that drive pathologic gene expression is a key challenge in precision oncology. Here, we have developed an analytic framework, named PRADA, that identifies oncogenic RNA-binding proteins through the systematic detection of coordinated changes in their target regulons. Application of this approach to data collected from clinical samples, patient-derived xenografts, and cell line models of colon cancer metastasis revealed the RNA-binding protein RBMS1 as a suppressor ...[more]

PMID: 32513775

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Interactome analysis of human RBMS1 protein in SW480 cells.

Project description:To identify protein binding partners of endogenous RBMS1, it was immunoprecipicated in triplicate, vs. IgG control, from SW480 colon adenocarcinoma cells, and subjected to MS analysis on an Q-exactive plus Orbitrap mass spectrometer. Raw files 13, 14, and 15 correspond to biological replicate IPs of RBMS1, and Raw files 16, 17, and 18 correspond to biological replicate IPs of IgG control.

2020-06-10 | PXD019479 | Pride

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