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Analysis of protein expression change upon RBMS1 knockdown

ABSTRACT: To assess how dpeletion of RBMS1 affects the proteome, SW480 colon adenocarcinoma cells expressing control or RBMS1 shRNAs were compared by Tandem Mass Tagging (TMT) protoemics.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Faraz Mardakheh

LAB HEAD: Faraz Mardakheh

PROVIDER: PXD019478 | Pride | 2020-06-10

REPOSITORIES: Pride

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RBMS1 Suppresses Colon Cancer Metastasis through Targeted Stabilization of Its mRNA Regulon.

Yu Johnny J Navickas Albertas A Asgharian Hosseinali H Culbertson Bruce B Fish Lisa L Garcia Kristle K Olegario John Paolo JP Dermit Maria M Dodel Martin M Hänisch Benjamin B Luo Yikai Y Weinberg Ethan M EM Dienstmann Rodrigo R Warren Robert S RS Mardakheh Faraz K FK Goodarzi Hani H

Cancer discovery 20200608 9

Identifying master regulators that drive pathologic gene expression is a key challenge in precision oncology. Here, we have developed an analytic framework, named PRADA, that identifies oncogenic RNA-binding proteins through the systematic detection of coordinated changes in their target regulons. Application of this approach to data collected from clinical samples, patient-derived xenografts, and cell line models of colon cancer metastasis revealed the RNA-binding protein RBMS1 as a suppressor ...[more]

PMID: 32513775

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