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CD63/CD9-GFP extracellular vesicles LC-MS/MS

ABSTRACT: This study aimed to identify subpopulations of extracellular vesicles (EVs) secreted by HeLa cells, and determine markers which enable to identify them, and which indicate their endosomal or plasma membrane origin. We used CD63 and CD9 as markers of EVs, and followed their intracellular trafficking using the RUSH assay. We used mass spectrometry to identify specific or common proteins in immunoprecipitated GFP+ EVs from HeLa transfected with the RUSH CD63-GFP or CD9-GFP, after a short (3h) or a long (24h) trafficking time from the endoplasmic reticulum.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Extracellular Vesicle, Cell Culture

SUBMITTER: Valentin SABATET

LAB HEAD: Damarys Loew

PROVIDER: PXD021515 | Pride | 2021-05-17

REPOSITORIES: Pride

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Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9.

Mathieu Mathilde M Névo Nathalie N Jouve Mabel M Valenzuela José Ignacio JI Maurin Mathieu M Verweij Frederik J FJ Palmulli Roberta R Lankar Danielle D Dingli Florent F Loew Damarys D Rubinstein Eric E Boncompain Gaëlle G Perez Franck F Théry Clotilde C

Nature communications 20210719 1

Despite their roles in intercellular communications, the different populations of extracellular vesicles (EVs) and their secretion mechanisms are not fully characterized: how and to what extent EVs form as intraluminal vesicles of endocytic compartments (exosomes), or at the plasma membrane (PM) (ectosomes) remains unclear. Here we follow intracellular trafficking of the EV markers CD9 and CD63 from the endoplasmic reticulum to their residency compartment, respectively PM and late endosomes. We ...[more]

PMID: 34282141

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