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Genetic variation of maize responses to low temperatures in the field

ABSTRACT: Eighteen genetically-diverse maize hybrids (Zea mays, dent lines crossed to a flint inbred-line) were cultivated in the field. Two conditions of cultivation were analyzed: normal sowing and early sowing. Upon early sowing plants grew under lower temperatures than upon normal sowing. Young leaves were harvested for proteomics quantitative analysis after a thermal time (equivalent number of days after emergence at 20°C) of 17 to 18. Three replicates per genotype/condition combination were analyzed. Each replicate was made up of the mix of leaf samples of five different plants.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Leaf

SUBMITTER: Thierry Balliau

LAB HEAD: Michel Zivy

PROVIDER: PXD021754 | Pride | 2021-01-19

REPOSITORIES: Pride

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Maize metabolome and proteome responses to controlled cold stress partly mimic early-sowing effects in the field and differ from those of Arabidopsis.

Urrutia Maria M Blein-Nicolas Mélisande M Prigent Sylvain S Bernillon Stéphane S Deborde Catherine C Balliau Thierry T Maucourt Mickaël M Jacob Daniel D Ballias Patricia P Bénard Camille C Sellier Hélène H Gibon Yves Y Giauffret Catherine C Zivy Michel M Moing Annick A

Plant, cell & environment 20210125 5

In Northern Europe, sowing maize one-month earlier than current agricultural practices may lead to moderate chilling damage. However, studies of the metabolic responses to low, non-freezing, temperatures remain scarce. Here, genetically-diverse maize hybrids (Zea mays, dent inbred lines crossed with a flint inbred line) were cultivated in a growth chamber at optimal temperature and then three decreasing temperatures for 2 days each, as well as in the field. Leaf metabolomic and proteomic profile ...[more]

PMID: 33410508

Similar Datasets

Genetic variation of maize responses to low temperatures in controlled conditions

Project description:Eighteen genetically-diverse maize hybrids (Zea mays, dent lines crossed to a flint inbred-line) were cultivated in a growth-chamber at optimal temperature (20°C) for three weeks. They were then submitted to three successive steps of decreasing temperature ( 16, 13 and 8.5°C). Each step lasted two days. Samples were taken on the youngest ligulated leaf (fourth or fifth leaf) at the end of the period at 20°C and at the end of each temperature step. Three replicates per genotype/ temperature combination were analyzed. Each replicate was made up of the mix of leaf samples from different plants.

2021-01-19 | PXD021790 | Pride

Genome-wide analysis of alternative splicing in Zea mays: landscape and genetic regulation

Project description:Analysis of the maize alternative splicing landscape, including transcript discovery and mapping of genotype-dependent variations in alternative splicing using B73, Mo17 and the SX19 inbred mapping population Total RNA was isolated from 5 week old leaves of hydroponically grown maize plants and used to construct RNA seq libraries

2014-10-29 | E-GEOD-57337 | biostudies-arrayexpress

arabidopsis ecotypes-Transcriptionnal response to potyviruses infection in Arabidopsis

Project description:ra06-05_potyvirus-ecotypes - arabidopsis ecotypes - Common and specific genes deregulated in response to various potyviruses in different Arabidopsis genetic backgrounds. - Four different Arabidopsis ecotypes were inoculated with one Potyviruse. About 4 weeks after sowing, the 6 expanded leaves plants were inoculated with the different viruses or were mock-inoculated. Seven days after inoculation, inoculated leaves were collected, RNA was extracted and virus infection controlled. RNA fron infected plants was then used for microarrays hybridization. Three biological repeat have been done and two dye-swap. Keywords: normal vs disease comparison

2008-10-20 | GSE10711 | GEO

Genome-wide expression of transcriptomes under waterlogging stress condition in subtropical maize

Project description:Genome-wide transcriptome analysis was performed to understand the expression pattern of transcriptomes in tolerant and susceptible subtropical maize genotypes under waterlogging stress condition. Waterlogging stress causes yield reduction in maize (Zea mays). It is important to dissect the genetic circuits that underlie the plant responses to waterlogging. So, the experiment was designed with the following objectives: to understand the expression pattern of transcriptomes in the tolerant and the susceptible genotypes under waterlogging stress; to identify DEGs functioning in important pathways underlying adaptive traits; to co-map bin locations of the transcriptomes with already known QTLs for waterlogging and find synteny with other species; and to generate gene co-expression networks to study cohorts of genes expressed together in modules and functional cluster, while comparing the two genotypes. Two tropical maize (Zea mays L.) inbred lines, HKI1105 (tolerant) and V-372 (susceptible), were used for our experiment. The genotypes were sown in plastic pots filled with loam soil with sandy texture. The plants were watered daily to soil capacity until the application of stress at 28 days after sowing. To impose waterlogging condition, the perforation in the bottom of the pots (35 cm in height) was sealed and the stress treatment was given by watering the pots (5 cm standing water) for seven continuous days. To allow for recovery from stress, the perforations of the pots were unsealed for proper drainage of excess water. The root samples from both genotypes were collected on the 28th, 32nd, 35th and 42nd day after sowing, which represented control, moderate stress, severe stress and post-stress recovery stages, respectively. Two biological replications were used for each comparison.

2013-08-14 | E-GEOD-43088 | biostudies-arrayexpress

An apoplastic peptide activates salicylic acid signaling in Zea mays

Project description:Papain-like cysteine proteases (PLCPs) play important roles in plant defense mechanisms. Previous work identified a set of five apoplastic PLCPs (CP1A, CP1B, CP2, XCP2 and CatB) which are crucial for the orchestration of SA-dependent defense signaling and vice versa in maize (Zea mays). One central question from these findings is which mechanism is triggered by apoplastic PLCPs to induce SA-dependent defenses. By a mass spectrometry approach we discovered a novel peptide (Zip1 = Zea mays immune signaling peptide) to be enriched in apoplastic fluid upon SA treatment. Zip1 induces PR-gene expression when applied to naїve maize leaves. Moreover, it activates apoplastic PLCPs similar as SA does, suggesting Zip1 to play an important role in SA-mediated defense signaling. In vitro studies using recombinant protein showed that CP1A and CP2, but not XCP2 and CatB, release Zip1 from its pro-peptide (PROZIP1) in vitro. Strikingly, metabolite analysis showed direct induction of SA de novo synthesis by Zip1 in maize leaves. In line with this, RNA sequencing revealed that Zip1-mediated changes in maize gene expression largely resemble SA-induced responses. Consequently, Zip1 increases maize susceptibility to the necrotrophic fungal pathogen Botrytis cinerea. In summary, this study identifies the PLCP-released peptide signal Zip1, which triggers SA signaling in maize.

2019-02-15 | PXD006751 | Pride

potyvirus effect-Transcriptionnal response to potyviruses infection in Arabidopsis

Project description:ra06-05_potyvirus-ecotypes - potyvirus effect - Common and specific genes deregulated in response to various potyviruses in different Arabidopsis genetic backgrounds. - Arabidopsis thaliana ecotype Ler was inoculated with three different Potyviruses. About 4 weeks after sowing, the 6 expanded leaves plants were inoculated with the different viruses or were mock-inoculated. Seven days after inoculation, inoculated leaves were collected, RNA was extracted and virus infection controlled. Three biological repeats have been done and two dye-swaps. Keywords: normal vs disease comparison

2008-10-20 | GSE10707 | GEO

Physiological and proteomic analyses indicate delayed sowing improves photosynthetic capacity in wheat flag leaves under heat stress

Project description:Background and aims Climate warming has become an indisputable fact, and wheat is among the most heat-sensitive cereal crops. Heat stress during grain filling threatens global wheat production and food security. Here, we analyzed the physiological and proteomic changes by delayed sowing on the photosynthetic capacity of winter wheat leaves under heat stress. Our aim is to provide a new cultivation way for the heat stress resistance in wheat. Methods Through 2 years field experiment and an open warming simulation system, we compared the changes in wheat grain weight, yield, photosynthetic rate, and chlorophyll fluorescence parameters under heat stress at late grain–filling stage during normal sowing and delayed sowing. At the same time, based on the iTRAQ proteomics, we compared the changes of differentially expressed proteins (DEPs) during the two sowing periods under high temperature stress.

2022-02-26 | PXD031871 | Pride

arabidopsis ecotypes-Transcriptionnal response to potyviruses infection in Arabidopsis

2010-05-18 | E-GEOD-10711 | biostudies-arrayexpress

affy_sunflower_2011_02-Effect of frost on sunflower

Project description:affy_sunflower_2011_02 - affy_sunflower_2011_02 - The early sowing constitutes an alternative strategy to avoid drought occurring during flowering and post-flowering periods and responsible for decrease in sunflower production. In French cropping system, early sowing is associated to low temperature period and frost during first development stages in sunflower. Knowledge about metabolism of frost acclimation must be performed to supply tools for breeding programs in sunflower. The aim of our experiment is to unravel the transcriptional regulation underpinning frost tolerance in sunflower-5 genotypes of sunflower were grown in a growth chamber 1 (23°C day/18°C night, 63% air humidity, 14 hours day photoperiod). At the stage of 6 leaves well-developed, 12 plants of each genotype were subjected to cold acclimation (+4°C during 2 days) in another growth chamber 2. Then, these plants were subjected to 2 nights at -3°C (frost treatment). Chlorophylle fluorescence, Osmotic potential, Relative electrolyte leakage were then determined in the following conditions : - 6th October on 6 plants X 5 genotypes from growth chamber 1 (C1) - 6th October on 6 plants X 5 genotypes from growth chamber 2 (S1) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (C2) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (S2)

2013-09-01 | GSE45586 | GEO

potyvirus effect-Transcriptionnal response to potyviruses infection in Arabidopsis

2010-05-18 | E-GEOD-10707 | biostudies-arrayexpress

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