Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic analysis reveals critical immunomolecular mechanisms in macrophage anti-tuberculosis processes

ABSTRACT: Tuberculosis infection activates the autoimmune system. However, the role of autoimmunity after Mycobacterium tuberculosis infection is unclear. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with tandem mass spectrometry (TMT) was used to perform proteomic analysis of five spinal tuberculosis and five protruding disc tissue samples. The identification of key molecular mechanisms provides a molecular target for future treatment of spinal tuberculosis, which may significantly improve patients' quality of life and prognosis.

INSTRUMENT(S): MaxQuant:LFQ intensity, 6410 Triple Quadrupole LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Intervertebral Disc

SUBMITTER: Xinli Zhan

LAB HEAD: Xinli Zhan

PROVIDER: PXD022541 | Pride | 2021-01-04

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Proteomic analysis reveals critical molecular mechanisms involved in the macrophage anti-spinal tuberculosis process.

Xu Guoyong G Xue Jiang J Jiang Jie J Liang Tuo T Yao Yuanlin Y Liao Shian S Chen Tianyou T Li Hao H Liu Chong C Zhan Xinli X

Tuberculosis (Edinburgh, Scotland) 20201209

Tuberculosis infection activates the autoimmune system. However, the role of host-pathogen interactions involved in Mycobacterium tuberculosis infection is unclear. In this study, we analyzed 6 spinal tuberculosis tissues and 6 herniated disc tissues by using liquid chromatography-tandem mass spectrometry coupled with tandem mass spectrometry, and immunohistochemical staining was performed for validating the results. We identified 42 differential immune-related proteins and 3 hub genes that are ...[more]

PMID: 33316736

Similar Datasets

N-terminus of Spt16 anchors FACT to Mcm2 for parental histone recycling

Project description:Parental histone recycling is essential for the restoration of chromatin-based epigenetic information during chromatin replication; however, the specific mechanisms underlying the local recycling of parental histones remain poorly understood. Here, we reveal an unexpected role of the Spt16-N domain in histone chaperone FACT during parental histone recycling and transfer in budding yeast. We found that depletion of Spt16 or mutations in the Spt16 middle domain leads to defects in both parental histone recycling and new histone deposition, affecting both the leading and lagging strands of DNA replication forks, highlighting the essential role of the FACT complex in both parental histone recycling and new histone deposition. Surprisingly, Spt16-N deletion results in an apparent defect in parental histone recycling, with a more pronounced defect on the lagging strand than the corresponding leading strand. Mechanistically, the Spt16-N domain acts as a protective barrier, shielding FACT-bound histone H3-H4 and facilitating its interaction with Mcm2, which ensures efficient local parental histone recycling. Collectively, the Spt16-N domain provides a protein–protein interaction module allowing FACT to act as a shuttle chaperone, cooperate with multiple replisome components, which act as co-chaperones, to form a complex involving the shuttle chaperone, histones, and co-chaperones, during parental histone recycling and transfer.

2024-01-26 | PXD043392 | Pride

DPAGT1-Mediated Protein N-Glycosylation Is Indispensable for Oocyte and Follicle Development in Mice

Project description:Posttranslational modification of proteins by N-linked glycosylation is crucial for many life processes. However, the exact contribution of N-glycosylation to mammalian female reproduction remains largely undefined. Here, DPAGT1, the enzyme that catalyzes the first step of protein N-glycosyation, is identified to be indispensable for oocyte development in mice. A recessive missense mutation (c. 497A>G; p. Asp166Gly) of Dpagt1 causes female subfertility without grossly affecting other functions. Mutant females ovulate fewer eggs owing to defects of follicular development beyond the late secondary stage. Oocytes ovulated by mutants carry a thin and fragile zona pellucida, and display poor developmental competence after fertilization in vitro. Moreover, completion of the first meiosis is accelerated in mutant oocytes, which is coincident with the elevation of aneuploidy. Mechanistically, transcriptomic analysis reveals the downregulation of a number of transcripts essential for oocyte meiotic progression and preimplantation development (e.g., Pttgt1, Esco2, Orc6, and Npm2) in mutant oocytes, which could account for the defects observed. Furthermore, conditional knockout (CKO) of Dpagt1 in oocytes recapitulates the phenotypes observed in Dpagt1 mutant females, and causes complete infertility. Taken together, these data indicate that protein N-glycosylation in oocytes is essential for female fertility in mammals by specific control of oocyte development.

2021-09-09 | PXD018531 | Pride

The YbjN regulon in Escherichia coli

Project description:YbjN, an enterobacteria-specific protein, is a multicopy suppressor of ts9 temperature sensitivity in Escherichia coli. Microarray study revealed that the expression level of ybjN was inversely correlated with the expression of flagellar, fimbrial and acid resistance genes. Over-expression of ybjN significantly down-regulated genes involved in the citric acid cycle, glycolysis, the glyoxylate shunt, oxidative phosphorylation, and amino acid and nucleotide metabolism. On the other hand, over-expression of ybjN up-regulated toxin-antitoxin modules, the SOS responsive pathway, cold shock proteins and starvation-induced transporter genes. Our results collectively suggest that YbjN may play important roles in regulating bacterial multicellular behaviors, metabolism and survival under various stress conditions in Es. coli. A total of 8 samples were analyzed: E. coli wild type strain (2 replicates); E. coli ybjN mutant strain (3 replicates); E. coli ybjN over-expression strain (3 replicates).

2011-10-14 | E-GEOD-28630 | biostudies-arrayexpress

Insulin reverses impaired alveolar fluid clearance in ARDS by inhibiting LPS-induced autophagy and inflammatory

Project description:Until now, acute respiratory distress syndrome (ARDS) has been a difficult clinical condition with a high mortality and morbidity rate, and is characterized by a build-up of alveolar fluid and impaired clearance. The underlying mechanism is not yet fully understood and no specific treatment available. Autophagy activation is associated with ARDS caused by different pathogenic factors. It represents a new direction of prevention and treatment of ARDS to restrain autophagy to a reasonable level through pharmacological and molecular genetic methods. Na, K-ATPase is the main gradient driver of pulmonary water clearance in ARDS and could be degraded by the autophagy-lysosome pathway to affect its abundance and enzyme activity. As a normal growth hormone in human body, insulin has been widely used in clinical for a long time. To investigate the association of insulin with Na, K-ATPase, autophagy and inflammatory markers in LPS-treated C57BL/6 mice by survival assessment, proteomic analysis, histologic examination, inflammatory cell counting, myeloperoxidase, TNF-α, IL-1β activity analysis etc. This was also verified on mouse alveolar epithelial type Ⅱ (AT Ⅱ) and A549 cells by transmission electron microscopy. We found that insulin restored the transport efficiency of Na, K-ATPase, inhibited the activation of autophagy and reduced the release of inflammatory factors caused by alveolar epithelial damage. The regulation mechanism of insulin on Na, K-ATPase by inhibiting autophagy function may provide new drug targets for the treatment of ARDS.

2023-08-13 | PXD040288 | Pride

MeDIP-chip from 3 pairs of gastric cancer and adjacent tissues

Project description:Methylated modifications of genome are common events in carcinogenesis and is involved in the tumorigenesis and progression of various cancers including gastric cancer Methylated DNA immunoprecipitation (MeDIP) combined with a human miRNA tiling microarray analysis demonstrated that there are much methylation differention between gastric cancers and adjacent controls microRNA gene methylation comparison of 3 pairs of gastric cancer and controls

2015-09-14 | E-GEOD-72979 | biostudies-arrayexpress

Analysis of circ101093 binding proteins in A549 cells after RNA pull-down.

Project description:We used circular RNA 101093 and its antisense to perform RNA pull-down, and analyzed the peptides in blank group, antisense group and circular RNA 101093 group. The object of this group is to analyze circ101093-binding protein. Blank and antisense group are used as negative control group.

2022-05-20 | PXD025575 | Pride

OsTZF1, a CCCH-Tandem Zinc Finger Protein gene, driven under own promoter produces no pleiotropic effects and confers salt tolerance in rice

Project description:The microarray was done using rice seedling plants grown in soil and treated with 250mM NaCl for two days.

2023-06-07 | E-MTAB-11842 | biostudies-arrayexpress

Transcript profiles of soybean organs exposed to dehydration

Project description:We used the microarray to identify dehydration-responsive genes of soybean organs.

2020-03-03 | E-MTAB-7010 | biostudies-arrayexpress

Transcription profiling by array of control and TCP13-SRDX transgenic Arabidopsis plants

Project description:To understand the downstream genes of TCP13, we performed analysis of gene expression using vector control plants and the TCP13 fused repression domain plants (TCP13pro::TCP13SRDX) . TheTCP13pro::TCP13SRDX plants is transgenic plants expressing chimeric repressor (SRDX) fused to TCP13 cDNA using the TCP13 promoter. TCP13 is a drought-inducible gene and play an essential role in leaf morphology. Arabidopsis plants were grown on the Murashige and Skoog (MS) medium (Murashige and Skoog, 1962), supplemented with 3% sucrose and 0.8 % agar (under a 16-h-day (60 ± 10 μmol photons m−2 s−1 light intensity) and 8-h-night regime for 2 weeks.

2023-07-07 | E-MTAB-9336 | biostudies-arrayexpress

Gm heat 30 min & 5 h

Project description:control plants vs heat treated plants (30 min & 5 h)

2015-08-12 | E-MTAB-2852 | biostudies-arrayexpress