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Identification of ESAG4-interacting proteins in Trypanosoma brucei bloodstream forms

ABSTRACT: How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. Flagellar cAMP signaling is implicated in interaction of the unicellular parasite Trypanosoma brucei with its hosts. The second messenger is produced by a large and atypical family of receptor-type adenylate cyclases (ACs) with unknown regulation mechanism. In order to identify trypanosome AC interacting proteins, ESAG4, an AC highly abundant in the parasite’s bloodstream stage, was fused to GFP and purified via GFP trap pull down followed by mass spectrometry.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Trypanosoma Brucei Brucei

SUBMITTER: Ignasi Forne

LAB HEAD: Boshart, Michael

PROVIDER: PXD025412 | Pride | 2022-08-31

REPOSITORIES: Pride

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A multi-adenylate cyclase regulator at the flagellar tip controls African trypanosome transmission.

Bachmaier Sabine S Giacomelli Giacomo G Calvo-Alvarez Estefanía E Vieira Larissa Rezende LR Van Den Abbeele Jan J Aristodemou Aris A Lorentzen Esben E Gould Matt K MK Brennand Ana A Dupuy Jean-William JW Forné Ignasi I Imhof Axel A Bramkamp Marc M Salmon Didier D Rotureau Brice B Boshart Michael M

Nature communications 20220916 1

Signaling from ciliary microdomains controls developmental processes in metazoans. Trypanosome transmission requires development and migration in the tsetse vector alimentary tract. Flagellar cAMP signaling has been linked to parasite social motility (SoMo) in vitro, yet uncovering control of directed migration in fly organs is challenging. Here we show that the composition of an adenylate cyclase (AC) complex in the flagellar tip microdomain is essential for tsetse salivary gland (SG) colonizat ...[more]

PMID: 36114198

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