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Stem cells from human exfoliated deciduous teeth cultured with human platelet lysate

ABSTRACT: Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients, especially fetal bovine serum, will increase the risk of zoonotic disease transmission during the use of animal-derived products. Human-platelet-lysate (hPL) was used in human cell expansion with reliability in clinical applications. This study aimed to explore the feasibility and effect of HPL in the cultivation of SHED. We use proteomics to interpret the impact of HPL on the protein profile of SHED. Propagation, differentiation, qPCR and immunofluorescence were used to detect the effect of HPL on SHED.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell

DISEASE(S): Disease Free

SUBMITTER: Tong Lei

LAB HEAD: Hongwu Du

PROVIDER: PXD026687 | Pride | 2022-05-19

REPOSITORIES: Pride

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Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells.

Lei Tong T Liu Yanyan Y Deng Shiwen S Xiao Zhuangzhuang Z Yang Yanjie Y Zhang Xiaoshuang X Bi Wangyu W Du Hongwu H

Journal of nanobiotechnology 20220402 1

Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients will increase the risk of zoonotic disease transmission. Human platelet lysate (HPL) is a potential surrogate and used in human cell expansion with reliability in clinical applications. In this study, we synthesized chitosan/gelatin/gellan gum hydrogel supplemented with HPL and investigated the effect of 3D culture for SHED. TMT-tagged proteomics was used to ...[more]

PMID: 35366889

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Single-cell transcriptome analysis of stem cells from human exfoliated deciduous teeth investigating functional heterogeneity in immunomodulation

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SHED: control vs Hepatocyte-like cells

Project description:Profiling of chromosomal gains and losses in a genomic complement of stem cells from human exfoliated deciduous teeth comparing control undifferentieated SHED with hepatocyte differentiated SHED.

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Effects of newly developed pulp-capping materials on early response of stem cells from exfoliated deciduous teeth: cell differentiation, migration, and proliferation based on next gene sequencing analysis

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Effects of prostate cancer patient urinary extracellular vesicles and hypoxic extracellular vesicles from prostate cancer cell lines on the transcriptomes of cancer-associated and normal fibroblasts

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Effects of urinary extracellular vesicles from prostate cancer patients on the transcriptomes of cancer-associated and normal fibroblasts

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