Phospho-proteomic analysis of DUSP6 deficient Caco-2 cells
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ABSTRACT: Dual-specificity phosphatase 6 (Dusp6)-deficiency enhances baseline colon barrier integrity and confers amelioration of dextran sulfate sodium (DSS)-induced colonic injury. DUSP6 is a phosphatase, and therefore it is plausible that DUSP6 knockout alters the phosphorylation status of other proteins. To access the protein phosphorylation landscape and functional network in DUSP6 deficient Caco-2 cells, we applied phosphopeptide enrichment and isobaric labeling strategies with MS-based proteomics, and identified 1323 phosphorylation sites on 580 phosphoproteins.
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Cell Culture
DISEASE(S): Colon Cancer
SUBMITTER: Chantal Hoi Yin Cheung
LAB HEAD: Hsueh-Fen Juan
PROVIDER: PXD029014 | Pride | 2021-11-02
REPOSITORIES: Pride
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