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Valvular interstitial cells and mesenchymal stem cell proteomes

ABSTRACT: Proteome profiling of valvular disease driver cell populations and mesenchymal stem cells undergoing osteogenic differentiation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Valve Cell, Aortic Valve

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Sasha Singh

LAB HEAD: Elena Aikawa

PROVIDER: PXD029949 | Pride | 2024-01-25

REPOSITORIES: Pride

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A disease-driver population within interstitial cells of human calcific aortic valves identified via single-cell and proteomic profiling.

Decano Julius L JL Iwamoto Yukio Y Goto Shinji S Lee Janey Y JY Matamalas Joan T JT Halu Arda A Blaser Mark M Lee Lang Ho LH Pieper Brett B Chelvanambi Sarvesh S Silva-Nicolau Jessica J Bartoli-Leonard Francesca F Higashi Hideyuki H Shibata Haruki H Vyas Payal P Wang Jianguo J Gostjeva Elena E Body Simon C SC Singh Sasha A SA Aikawa Masanori M Aikawa Elena E

Cell reports 20220401 2

Cellular heterogeneity of aortic valves complicates the mechanistic evaluation of the calcification processes in calcific aortic valve disease (CAVD), and animal disease models are lacking. In this study, we identify a disease-driver population (DDP) within valvular interstitial cells (VICs). Through stepwise single-cell analysis, phenotype-guided omic profiling, and network-based analysis, we characterize the DDP fingerprint as CD44highCD29+CD59+CD73+ ...[more]

PMID: 35417712

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