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HOXB13 WT and G84E mass spectrometry

ABSTRACT: To identify potential cofactors of HOXB13 in suppressing lipogenic programs in prostate cancer cells, we performed tandem affinity purification followed by mass spectrometry analysis of WT and G84E HOXB13 expressed in LNCaP cells. Out of the HOXB13-enriched proteins are previously reported interactors such as AR and its cofactors FOXA1, GATA2, and NKX3. However, these interactions were not disrupted by G84E as compared to WT HOXB13. Interestingly, we found strong interactions of HOXB13 with HDAC1/3 and their corepressors NCoR1/2 and TBL1X. Notably, these interactions were drastically reduced by G84E mutation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Xiaodong Lu

LAB HEAD: Jindan Yu

PROVIDER: PXD030810 | Pride | 2023-03-11

REPOSITORIES: Pride

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			Action	DRS
	JYMS031_HOXB13_WT.pep.xml	Pepxml
	JYMS031_HOXB13_WT.raw	Raw
	JYMS032_HOXB13_G84E.pep.xml	Pepxml
	JYMS032_HOXB13_G84E.raw	Raw
	checksum.txt	Txt

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Publications

The Chemical Synthesis of Insulin: An Enduring Challenge.

Karas John A JA Wade John D JD Hossain Mohammed Akhter MA

Chemical reviews 20210309 8

The pancreatic peptide hormone insulin, first discovered exactly 100 years ago, is essential for glycemic control and is used as a therapeutic for the treatment of type 1 and, increasingly, type 2 diabetes. With a worsening global diabetes epidemic and its significant health budget imposition, there is a great demand for new analogues possessing improved physical and functional properties. However, the chemical synthesis of insulin's intricate 51-amino acid, two-chain, three-disulfide bond struc ...[more]

PMID: 33689304

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