Dataset Information
Co-immunoprecipitation of proteins with WT HIV-1 IN, K258R HIV-IN, and K258/264/266/273R
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
Shelby Winans
LAB HEAD: Stephen P. Goff
PROVIDER: PXD031825 | Pride | 2022-05-20
REPOSITORIES: Pride
Publications
A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats.
Nature communications 20220318 1
Retroviruses utilize the viral integrase (IN) protein to integrate a DNA copy of their genome into host chromosomal DNA. HIV-1 integration sites are highly biased towards actively transcribed genes, likely mediated by binding of the IN protein to specific host factors, particularly LEDGF, located at these gene regions. We here report a substantial redirection of integration site distribution induced by a single point mutation in HIV-1 IN. Viruses carrying the K258R IN mutation exhibit a high fre ...[more]
