Investigating the monomer-dimer transition of PKD1 kinase domain with EDC crosslinking

ABSTRACT: The project aim was to generate experimental constraints for the in silico modeling of the dimeric structure of the PKD kinase domain. And identify conformational changes upon dimerization. For this we used the heterobifunctional zero length crosslinker EDC to identify salt bridges within the protein and between the molecules in the dimeric arrangement. We could observe a stabilization of the protein in particularly in the activation loop upon dimerization and successfully identified one abundant dimer specific crosslink that helped us to identify contact surfaces in the dimer interface.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: WeiQiang Chen  

LAB HEAD: Thomas Leonard

PROVIDER: PXD031997 | Pride | 2023-03-11

REPOSITORIES: Pride