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Translation regulation mechanisms in astrocytes

ABSTRACT: Identifying the proteins associated with the polyribosomes in astrocytes from the mouse brain to unveil translation regulation mechanisms in these cells.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Astrocyte

SUBMITTER: Reda Mekdad

LAB HEAD: Damarys Loew

PROVIDER: PXD033121 | Pride | 2023-07-20

REPOSITORIES: Pride

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The ribosome-associated protein RACK1 represses Kir4.1 translation in astrocytes and influences neuronal activity.

Oudart Marc M Avila-Gutierrez Katia K Moch Clara C Dossi Elena E Milior Giampaolo G Boulay Anne-Cécile AC Gaudey Mathis M Moulard Julien J Lombard Bérangère B Loew Damarys D Bemelmans Alexis-Pierre AP Rouach Nathalie N Chapat Clément C Cohen-Salmon Martine M

Cell reports 20230430 5

The regulation of translation in astrocytes, the main glial cells in the brain, remains poorly characterized. We developed a high-throughput proteomics screen for polysome-associated proteins in astrocytes and focused on ribosomal protein receptor of activated protein C kinase 1 (RACK1), a critical factor in translational regulation. In astrocyte somata and perisynaptic astrocytic processes (PAPs), RACK1 preferentially binds to a number of mRNAs, including Kcnj10, encoding the inward-rectifying ...[more]

PMID: 37126448

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