Vap33 protein interactome - The Drosophila tumor suppressors Lgl and Vap33 activate the Hippo pathway by a dual mechanism involving RtGEF/Git/Arf79F and inhibition of the V-ATPase

ABSTRACT: To identify Vap33 binding proteins, we purified Vap33-containing protein complexes from Drosophila embryos, using the single-step GFP-trap bead purification from a fly line expressing YFP-tagged Vap33, followed by nanoLC-MS/MS analysis of the interactors. This study revealed the binding of Vap33 to RtGEF and Git, which we further connected to the regulation of Hippo signaling.

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Alexey Veraksa  

LAB HEAD: Alexey Veraksa

PROVIDER: PXD035110 | Pride | 2024-02-01

REPOSITORIES: Pride