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Vap33 protein interactome - The Drosophila tumor suppressors Lgl and Vap33 activate the Hippo pathway by a dual mechanism involving RtGEF/Git/Arf79F and inhibition of the V-ATPase

ABSTRACT: To identify Vap33 binding proteins, we purified Vap33-containing protein complexes from Drosophila embryos, using the single-step GFP-trap bead purification from a fly line expressing YFP-tagged Vap33, followed by nanoLC-MS/MS analysis of the interactors. This study revealed the binding of Vap33 to RtGEF and Git, which we further connected to the regulation of Hippo signaling.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Alexey Veraksa

LAB HEAD: Alexey Veraksa

PROVIDER: PXD035110 | Pride | 2024-02-01

REPOSITORIES: Pride

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The Drosophila tumour suppressor Lgl and Vap33 activate the Hippo pathway through a dual mechanism.

Portela Marta M Mukherjee Swastik S Paul Sayantanee S La Marca John E JE Parsons Linda M LM Veraksa Alexey A Richardson Helena E HE

Journal of cell science 20240216 4

The tumour suppressor, Lethal (2) giant larvae [Lgl; also known as L(2)gl], is an evolutionarily conserved protein that was discovered in the vinegar fly Drosophila, where its depletion results in tissue overgrowth and loss of cell polarity. Lgl links cell polarity and tissue growth through regulation of the Notch and the Hippo signalling pathways. Lgl regulates the Notch pathway by inhibiting V-ATPase activity via Vap33. How Lgl regulates the Hippo pathway was unclear. In this current study, we ...[more]

PMID: 38240353

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