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Loss of centrosomal gene ALMS1 alters lipid metabolism and the regulation of processes linked to the extracellular matrix.

ABSTRACT: In this project we evaluated the proteomic profiling with TGF-β stimuli at 24h in a CRISPR-Cas9 model for ALMS1 gene in hTERT-BJ-5ta cells. Proteomic results showed a majority inhibition of downstream regulated pathways by the TGF-β, associating the protein coding genes (PCG) with processes like TGF- β matrix regulation, epithelial mesenchymal transition (EMT), PI3K/AKT or P53. In conclusion, seems that the depletion of ALMS1 could be inhibiting the signals transduction through the TGF -β and the routes regulated downstream.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte, Cell Culture

DISEASE(S): Ciliopathy

SUBMITTER: Brais Bea Mascato

LAB HEAD: Brais Bea-Mascato

PROVIDER: PXD035708 | Pride | 2024-01-26

REPOSITORIES: Pride

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			Action	DRS
	DPGDVP340140.pptx	Other
	DPGDVP340140_3525_proteins.xlsx	Xlsx
	RAW_DATA_DPGDVP340140.rar	Other
	checksum.txt	Txt

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CRISPR-Cas9-mediated ALMS1 knockout inhibits TGF-β signalling and epithelial-mesenchyme transition.

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