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Two elongasomes generate rod shape in a unicellular microorganism

ABSTRACT: Cell geometry is genetically inherited by all forms of life. In bacteria, shape is determined by the peptidoglycan and is nearly invariant during the metabolically active vegetative state. Essential morphogenetic complexes, the elongasome and divisome, expand the peptidoglycan along the major and minor axes, respectively, to generate a particular shape. Here, we provide evidence for the presence in Salmonella of two alternative elongasomes that generate a similar (rod) shape. One elongasome is directed by penicillin-binding protein 2 (PBP2) while the other is controlled by PBP2SAL, a pathogen-specific enzyme. In acidic niches, PBP2SAL replaces and renders dispensable PBP2. Our data also demonstrate distinct rates for the two identified elongasomes, with that directed by PBP2SAL exhibiting lower speed

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. Sl1344

SUBMITTER: Alberto Paradela

LAB HEAD: Francisco Garcia del Portillo

PROVIDER: PXD039436 | Pride | 2023-08-15

REPOSITORIES: Pride

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Evidence of two differentially regulated elongasomes in Salmonella.

Castanheira Sónia S García-Del Portillo Francisco F

Communications biology 20230909 1

Cell shape is genetically inherited by all forms of life. Some unicellular microbes increase niche adaptation altering shape whereas most show invariant morphology. A universal system of peptidoglycan synthases guided by cytoskeletal scaffolds defines bacterial shape. In rod-shaped bacteria, this system consists of two supramolecular complexes, the elongasome and divisome, which insert cell wall material along major and minor axes. Microbes with invariant shape are thought to use a single morpho ...[more]

PMID: 37689828

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