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Systematic characterization of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry (HEK293)

ABSTRACT: To improve understanding of the role of site-specific proline hydroxylation in controlling protein function, we have developed a robust workflow for the identification of proline hydroxylation sites in proteins using a combination of hydrophilic interaction chromatography (HILIC) enrichment and high-resolution nano-Liquid Chromatography-Mass Spectrometry (LC-MS). Using this approach, together with refining and filtering parameters during data analysis, by combining the results from cell lines being treated with either the prolyl hydroxylase inhibitor Roxadustat (FG-4592, FG) or the proteasome inhibitor MG-132 (MG), or DMSO, a total of 4,993 and 3,247 proline hydroxylation sites were identified in HEK293 (PT10822) and RCC4 (PT10402) samples.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hao Jiang

LAB HEAD: Angus Lamond

PROVIDER: PXD044663 | Pride | 2025-11-28

REPOSITORIES: Pride

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			Action	DRS
	DMSO_HILIC_F1.raw	Raw
	DMSO_HILIC_F10.raw	Raw
	DMSO_HILIC_F11.raw	Raw
	DMSO_HILIC_F12.raw	Raw
	DMSO_HILIC_F13.raw	Raw

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Systematic characterization of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry (RCC4)

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