Proteomics

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Integrated proteomics reveals autophagy degradation landscape in neurons and receptors regulating neuronal activity

ABSTRACT: Autophagy is a constitutive process of lysosomal degradation required for maintaining the homeostasis of large molecular complexes and organelles in neurons. Here we reported a systemic investigation of neuronal autophagy targets through integrated proteomics and functional analysis. Proteomic profiling of human and mouse neurons with autophagy inhibition and LC3-interactome reveals a role for neuronal autophagy in targeting a wide range of cellular pathways and organelles. Our study reveals the landscape of autophagy degradation in neurons and insight into mechanisms of neurological disorders linked to autophagy deficiency.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Brain, Stem Cell

SUBMITTER: Haiyan Tan  

LAB HEAD: Junmin Peng

PROVIDER: PXD048730 | Pride | 2024-06-22

REPOSITORIES: Pride

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Publications

Integrated proteomics reveals autophagy landscape and an autophagy receptor controlling PKA-RI complex homeostasis in neurons.

Zhou Xiaoting X   Lee You-Kyung YK   Li Xianting X   Kim Henry H   Sanchez-Priego Carlos C   Han Xian X   Tan Haiyan H   Zhou Suiping S   Fu Yingxue Y   Purtell Kerry K   Wang Qian Q   Holstein Gay R GR   Tang Beisha B   Peng Junmin J   Yang Nan N   Yue Zhenyu Z  

Nature communications 20240410 1

Autophagy is a conserved, catabolic process essential for maintaining cellular homeostasis. Malfunctional autophagy contributes to neurodevelopmental and neurodegenerative diseases. However, the exact role and targets of autophagy in human neurons remain elusive. Here we report a systematic investigation of neuronal autophagy targets through integrated proteomics. Deep proteomic profiling of multiple autophagy-deficient lines of human induced neurons, mouse brains, and brain LC3-interactome reve  ...[more]

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