Dataset Information

N-terminal processing of CSP in P. falciparum salivary gland sporozoites

ABSTRACT: Salivary gland sporozoites of the malaria parasite Plasmodium express a major surface coat protein, CSP. The N-terminus of this protein is hypothesized to be enzymatically processed, revealing a Gln residue which is then enzymatically converted pyro-Glu. We analyzed P. falciparum salivary gland sporozoites, recombinant PfCSP, and synthetic peptides in order to characterize this cleavage site and its conversion.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Plasmodium Falciparum Nf54

DISEASE(S): Plasmodium Falciparum Malaria

SUBMITTER: Kristian Swearingen

LAB HEAD: Kristian Swearingen

PROVIDER: PXD052186 | Pride | 2025-05-07

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2024-02-27_1hDDA_PfSGspz_heatCleave_01.interact.pep.mzid	Mzid
	2024-02-28_1hDDA_PfSGspz_heatCleave_02.interact.pep.mzid	Mzid
	2024-02-28_1hDDA_PfSGspz_heatCleave_03.interact.pep.mzid	Mzid
	CSP_peptide_Spectra_with_USIs.xlsx	Xlsx
	Description_of_Sample_Files.xlsx	Xlsx

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Publications

Protective antibodies target cryptic epitope unmasked by cleavage of malaria sporozoite protein.

Dacon Cherrelle C Moskovitz Re'em R Swearingen Kristian K Da Silva Pereira Lais L Flores-Garcia Yevel Y Aleshnick Maya M Kanatani Sachie S Flynn Barbara B Molina-Cruz Alvaro A Wollenberg Kurt K Traver Maria M Kirtley Payton P Purser Lauren L Dillon Marlon M Bonilla Brian B Franco Adriano A Petros Samantha S Kritzberg Jake J Tucker Courtney C Paez Gonzalo Gonzalez GG Gupta Priya P Shears Melanie J MJ Pazzi Joseph J Edgar Joshua M JM Teng Andy A AA Belmonte Arnel A Oda Kyosuke K Doumbo Safiatou S Krymskaya Ludmila L Skinner Jeff J Li Shanping S Ghosal Suman S Kayentao Kassoum K Ongoiba Aissata A Vaughan Ashley A Campo Joseph J JJ Traore Boubacar B Barillas-Mury Carolina C Wijayalath Wathsala W Idris Azza A Crompton Peter D PD Sinnis Photini P Wilder Brandon K BK Zavala Fidel F Seder Robert A RA Wilson Ian A IA Tan Joshua J

Science (New York, N.Y.) 20250103 6729

The most advanced monoclonal antibodies (mAbs) and vaccines against malaria target the central repeat region or closely related sequences within the <i>Plasmodium falciparum</i> circumsporozoite protein (PfCSP). Here, using an antigen-agnostic strategy to investigate human antibody responses to whole sporozoites, we identified a class of mAbs that target a cryptic PfCSP epitope that is only exposed after cleavage and subsequent pyroglutamylation (pGlu) of the newly formed N terminus. This pGlu-C ...[more]

PMID: 39745947

Similar Datasets

Project description:Simultaneous analysis of Plasmodium falciparum and Anopheles gambiae gene expression: an assessment of parasite maturation and of the mosquito response to infection. Malaria is probably the most lethal and most prevalent parasitic disease in the world, causing more than one million deaths per year. In spite of intensive research, no vaccine exists and good vaccine candidates are still a necessity. Plasmodium sporozoites from mosquito midguts are not as infective as those from salivary glands, presenting a different type of motility which is presumably associated with their invasive capacity. It is assumed that these differences are due to maturation of sporozoites, which happens either in the salivary glands or somewhere along the way from the midgut to the glands. Indeed, the invasion of the salivary gland is considered to be an essential step for malaria transmission to the host and it is known to causes expression of multiple genes in the parasite and activation of some immune-response genes in the insect. Here we compared the gene expression profile of P. falciparum parasites from midgut and salivary glands in order to search for genes involved in parasite maturation; on the assumption that any differentially expressed genes found under this condition could be potentially related to the higher infectivity of the salivary gland derived sporozoites and therefore would be potentially good vaccine candidates. Also, because very little is known about mosquito genes involved in the response to Plasmodium, we compared the gene expression profile of infected and uninfected midguts and salivary glands. Our results demonstrate that, upon infection, at least 13 genes are differentially expressed in the mosquito midgut and 43 in the salivary gland. Gene ontology enrichment analysis showed that among other things, in mosquito salivary glands, infection causes multiple genes related to chitin metabolism to be differentially expressed. Also and more importantly, at least 54 genes were differentially expressed in P. falciparum from salivary glands in comparison to midgut (19 down-regulated and 35 up-regulated). Last, we show for the first time that P. falciparum causes multiple genes in the salivary gland to be regulated and that some of those genes could be potentially beneficial to the parasite.