PUS7 cytoplasmic localization directs a pseudouridine-mediated cellular stress response

ABSTRACT: Pseudouridine (Ψ) is an abundant post-transcriptional modification found across all classes of RNA. Since its discovery in mRNAs a decade ago, it has been widely speculated that Y might govern additional post-transcriptional regulation of gene expression. Here, we demonstrate that one of the principal enzymes responsible for adding Y to mRNAs, pseudouridine synthase 7 (PUS7), accumulates in the cytoplasm under a variety of stress conditions in Saccharomyces cerevisiae and BEAS-2B human epithelial lung cells. The localization of PUS7 to the cytoplasm promotes Y-incorporation into hundreds of different mRNA sequences and increases cellular fitness under ROS and divalent metal ion stress. The preponderance of newly identified Y-sites lies within portions of the mRNA important for post-transcriptional control—-coding regions and 3’ UTRs. Quantitative proteomics reveal that shifts in the cellular post-transcriptional modification landscape upon PUS7 relocalization reshapes the proteome. Our data suggest a mechanism whereby stressors localize PUS7 in the cytoplasm to enable the direct modification and regulation of stress response mRNAs, thereby protecting cells from further stress-induced damage. (2A-PUS7-control682832B-PUS7-control682842C-PUS7-control682854A-PUS7-NES-Co682864A-PUS7-NES-Co682874B-PUS7-NES-Co682885A-PUS7-control-Co682895B-PUS7-control-Co682905C-PUS7-control-Co68291PUS7-NES_CU_1A 75059PUS7-NES_CU_1B 75060PUS7-NES_CU_1C 75061PUS7-NLS_CU_2A 75062PUS7-NLS_CU_2B 75063PUS7-NLS_CU_2C 75064PUS7-WT_CU_3A 75065PUS7-WT_CU_3B 75066PUS7-WT_CU_3C 75067)

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae By4741

SUBMITTER: Minli Ruan  

LAB HEAD: Kristin Koutmou

PROVIDER: PXD052684 | Pride | 2026-01-28

REPOSITORIES: Pride