Identification of deubiquitinases controlled by extracellular matrix stiffness in cancer cells

ABSTRACT: The project was aimed at identifying deubiquitinases (DUBs) involved in cancer cell mechanosignaling. Lysates from cancer cells grown on collagen matrices with varying stiffnesses were incubated with an activity-based HA-tagged ubiquitin probe that covalently binds DUB active site. To identify DUBs differentially activated on soft and stiff collagen hydrogels, lysates were immunoprecipitated with anti-HA agarose beads. Immunoprecipitates were further identified by LC-MS/MS analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte

DISEASE(S): Skin Cancer

SUBMITTER: AUDEBERT Stephane  

LAB HEAD: DECKERT Marcel, PhD

PROVIDER: PXD054010 | Pride | 2025-08-22

REPOSITORIES: Pride