Chromatin cell cycle proteomics reveals nuclear metabolism

ABSTRACT: The progression of the cell cycle is regulated by the expression of specific genes and fluctuations in cellular metabolic states. By conducting cell cycle-based transcriptomics, proteomics, and metabolomics analyses, previous research has identified cell cycle-dependent alterations at the molecular, protein, and metabolic levels. However, the significance of protein compartmentalization, coupled with the evidence that metabolic enzymes can localize to the nucleus and influence chromatin states, suggests that fluctuations in nuclear metabolism may play a role in regulating cell cycle progression. In this study, we present a systematic approach to investigating the chromatin environment at a cell cycle resolution. By focusing on metabolic enzymes, we were able to ascertain that phosphatidylinositol metabolism localizes to the nucleus and undergoes alterations throughout the cell cycle. Moreover, the inhibition of phosphatidylinositol metabolism affects histone methylation in a manner that is dependent on the cell cycle, which ultimately impairs cell cycle progression. Our study demonstrates that nuclear metabolism is a dynamic process that shapes the chromatin environment and establishes a direct connection between nuclear metabolism and the cell cycle.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Savvas Kourtis  

LAB HEAD: Sara Sdelci

PROVIDER: PXD055357 | Pride | 2026-06-29

REPOSITORIES: pride