Proteomics

Dataset Information

0

Interaction of saposin C and acid-beta-glucosidase

ABSTRACT: The objective of this project was to determine the interaction interface(s) between acid beta-glucosidase (GCase) and its activator protein saposin C (SapC). Chemical crosslinking was used to confirm the formation of discrete heterodimers between the two proteins, using lysine-reactive crosslinkers BS3 and DTSSP and both non-activating and activating buffer systems. High-resolution mass spectrometry was then used to determine the sites of crosslinking, which informed the modes of protein-protein interactions. We identified two binding surfaces between GCase and saposin C using this workflow.

INSTRUMENT(S): Orbitrap Exploris 480, Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Lysosomal Storage Disease

SUBMITTER: Minh Thu Ma  

LAB HEAD: Raquel Lieberman

PROVIDER: PXD058056 | Pride | 2025-05-07

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20240529_DH_14_Gel2_h.pdResult Other
20240529_DH_14_Gel2_h.raw Raw
20240529_DH_14_Gel2_h.xlsx Xlsx
20240529_DH_4_Gel1_f.pdResult Other
20240529_DH_4_Gel1_f.raw Raw
Items per page:
1 - 5 of 30
altmetric image

Publications

Targeting proteins to their final cellular destination requires transport mechanisms and nearly all lysosomal enzymes reach the lysosome via the mannose-6-phosphate receptor pathway. One of the few known exceptions is the enzyme β-glucocerebrosidase (GCase) that requires the lysosomal integral membrane protein type-2 (LIMP-2) as a proprietary lysosomal transporter. Genetic variations in the GCase encoding gene GBA1 cause Gaucher's disease (GD) and present the highest genetic risk factor to devel  ...[more]

Similar Datasets

Proteomics Higher-order structure and proteoforms of co-occurring C4b-binding protein assemblies in human serum
2024-05-21 | PXD047679 | Pride
Proteomics Mechanism of 60S ribosomal subunit recognition and UFMylation by the UFM1 E3 ligase complex
2024-03-22 | PXD046990 | Pride
Proteomics Monolinks Matter: Rational Modification of a Cross-Linker for Improved Flexible Protein Structure Modeling
2025-05-06 | PXD051742 | Pride
Proteomics Aberrant troponin post-translational modifications with TNNT1 mutations
2025-01-08 | PXD054504 | Pride
Proteomics A SAM key domain required for enzymatic activity and allosteric activation of the Fun30 nucleosome remodeler
2023-07-18 | PXD037249 | Pride
Proteomics Histone interaction landscapes visualized by crosslinking mass spectrometry in intact cell nuclei
2018-07-23 | PXD007513 | Pride
Proteomics HAP40 orchestrates huntingtin structure for differential interaction with polyglutamine expanded exon 1
2022-09-09 | PXD028415 | Pride
Proteomics HAP40 orchestrates huntingtin structure for differential interaction with polyglutamine expanded exon 1
2022-02-21 | PXD028313 | Pride
Proteomics IP-to-MS: an unbiased workflow for antigen profiling
2025-05-07 | PXD056617 | Pride
Proteomics Human cell surface-AAV interactomes identify LRP6 as blood-brain-barrier transcytosis receptor and immune cytokine IL3 as AAV9 binder
2024-07-31 | PXD045380 | Pride