Confirmation of the phosphorylation sites of human PhK

ABSTRACT: The activity of PhK is regulated by phosphorylation, and we utilized single-particle cryo-electron microscopy to solve the high-resolution structures of human PhK under phosphorylation and calcium ion activation. To gain a deeper insight into the phosphorylation sites following PKA treatment, we employed tandem mass spectrometry for precise localization of the phosphorylation sites within PhK.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Ruifang Ma  

LAB HEAD: Kaige Yan

PROVIDER: PXD059345 | Pride | 2025-05-07

REPOSITORIES: Pride