Identification of RSRC2 interactome through Immunoprecipitation (IP) coupled with LC-MS/MS-based protein analysis

ABSTRACT: We immunoprecipitate (IP) RSRC2 from HCT116 cells in the presence or absence of RNase A/T1 mix to identify RNA-mediated and RNA-independent RSRC2 protein-protein interaction networks. Four biological replicates were performed for each IP-MS in cells +/- RNase A/T1 Mix (EN0551, ThermoFisher) using IgG and RSRC2 antibodies. Extracted proteins from RNase A/T1 treated and untreated cells (4 replicates per condition) were compared using label-free (LFQ) Quantitative proteomics.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Faraz Mardakheh  

LAB HEAD: Faraz Mardakheh

PROVIDER: PXD059507 | Pride | 2026-03-12

REPOSITORIES: pride