Dataset Information

Diversity and Heterogeneity of Extracellular Vesicles and Brain-Penetrating Amembranous Non-Vesicular Nanoparticles in Glioblastoma

ABSTRACT: Advances in understanding intercellular communication through extracellular vesicles (EVs) and non-vesicular extracellular nanoparticles (NVEPs) have been limited by their cellular heterogeneity and unclear origins. Using sequential ultracentrifugation, we identified high- and low-density EVs and performed multi-omics analyses on four EV/NVEP categories, including supermeres. Our findings reveal that extracellular RNA, RNA-binding proteins, and other cellular proteins are differentially expressed among EVs and NVEPs. Notably, supermeres can penetrate the blood-brain barrier, in a manner dependent on their intact RNA and protein structure, and distribute throughout the CNS. Supermeres preferentially interact with microglia at higher levels than other CNS cell types. These interactions lead to decreased TGFβ expression, suggesting an immunomodulatory role. This study highlights supermeres as a promising platform for CNS therapeutic delivery.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Joao Paulo

LAB HEAD: Alain Charest

PROVIDER: PXD059671 | Pride | 2025-10-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ACsamplelist_final.xlsx	Xlsx
	a20599.raw	Raw
	a20599_XL_HMFP_Exosome_20201221.mzIdentML	Mzid
	a20600.raw	Raw
	a20600_XL_HMFP_Exosome_20201221.mzIdentML	Mzid

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Publications

Heterogeneity of Extracellular Vesicles and Non-Vesicular Nanoparticles in Glioblastoma.

Xu Tuoye T Paulo Joao A JA Zhang Piyan P Liu Xinyue X Nguyen Alya A Cheng Yuanhua Y Massick Clark C Zhang Yanhong Y Jeppesen Dennis K DK Zhang Qin Q Higginbotham James N JN Tutanov Oleg S OS Krichevsky Anna M AM Chiu Daniel T DT Gygi Steve P SP Vickers Kasey C KC Franklin Jeffrey L JL Coffey Robert J RJ Charest Al A

Journal of extracellular vesicles 20251001 10

It is increasingly clear that intercellular communication is largely mediated by lipid-bilayer, membrane-bound extracellular vesicles (EVs) and amembranous, non-vesicular extracellular particles (NVEPs), including exomeres and the recently identified supermeres. To elucidate the cargo and functional roles of these carriers, we performed a comprehensive analysis of their lipid, protein and RNA content in the context of colorectal cancer and glioblastoma (GBM). Our results demonstrate that EVs exh ...[more]

PMID: 41039818

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