Dataset Information

Proteome of DiFi and CC-CR supermeres purified by FPLC with or without additional affinity purification for TGFBi

ABSTRACT: The cell seretome is diverse and contains extracellular secreted vesicles (EVs) as well as non-vesicular extracellular nanoparticles (NVEPs) called exomeres and supermeres, all of which carry different kinds of cell signaling cargos. One supermere enriched cargo is transforming growth factor beta induced (TGFBi) protein. We have created TGFBi-neon green-His tagged expressing cell lines in the microsatellite stable (MSS) colorectal cancer cell line DiFi and another one in CC-CR cells with microsatellite instability (MSI). We have developed a fast protein liquid chromatography (FPLC) method for isolating EVs, exomeres and supermeres from biofluids. We used either FPLC or FPLC and then nickel columns that bind His tags to isolate supermeres from DiFi or CC-CR cells. We utilized a proteomics approach acquiring LC-MS/MS data to compare FPLC to FPLC and His tagged TGFBi-containing purified supermeres from DiFi and CC-CR cells. This was done using hollow fiber 3D bioreactor production of conditioned media from these cell lines.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Colon

DISEASE(S): Colon Cancer

SUBMITTER: Kristie Lindsey Rose

LAB HEAD: Kristie Lindsey Rose

PROVIDER: PXD066888 | Pride | 2025-09-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Protein_Report_Proj6225.xls	Xls
	Spectrum_Report_Proj6225.xlsx	Xlsx
	pQE4_ml_6225_20230203_S1_DiFi_FPLC_2uL_1.raw	Raw
	pQE4_ml_6225_20230203_S2_DiFi_His_2uL_2.raw	Raw
	pQE4_ml_6225_20230203_S3_CCCR_FPLC_2uL_3.raw	Raw

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Proteome of DiFi and CC-CR supermeres purified by FPLC with or without additional affinity purification for TGFBi

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