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TCA rewiring underpins implantation and acetylome programming

ABSTRACT: Metabolism has emerged as a crucial regulator of development and stem cell epigenome. This coupling is particularly evident during the in vitro exit from naïve pluripotency. However, our understanding of the dynamic metabolic rewiring at this developmental stage remains rudimentary. In this study, we reconstruct intracellular metabolite routings in pre- and post-implantation mouse embryos and their dynamic stem cell models.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Roberta Noberini

LAB HEAD: Tiziana Bonaldi

PROVIDER: PXD062776 | Pride | 2026-06-08

REPOSITORIES: Pride

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			Action	DRS
	08-2025.zip	Other
	Eleni.zip	Other
	Exp_design_2.xlsx	Xlsx
	P250806_RN_Eleni01.raw	Raw
	P250806_RN_Eleni02.raw	Raw

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Publications

TCA cycle rewiring underpins histone acetylation sourcing and cell-fate transitions during exit from naive pluripotency.

Kafkia Eleni E Pladevall-Morera David D Argemi-Muntadas Lidia L Wang Gangqi G Noberini Roberta R Casòliba-Melich Arnau A Bagés-Arnal Sandra S Anagho-Mattanovich Matthias M Silvério-Alves Rita R Gassler Johanna J Bonaldi Tiziana T Rabelink Ton J TJ Moritz Thomas T Żylicz Jan Jakub JJ

Cell stem cell 20260424 5

Metabolism shapes stem cell differentiation and epigenome regulation, especially during the exit from naive pluripotency in vitro. Yet how metabolic networks reorganize at implantation remains unclear. Here, we map metabolite routing in pre- and post-implantation mouse embryos and across dynamic pluripotency transitions in stem cells, revealing that the tricarboxylic acid (TCA) cycle undergoes spatio-temporal rewiring rather than a simple shutdown. Pyruvate emerges as a central metabolic nexus, ...[more]

PMID: 42034059

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