Proteomics

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Proximity labeling of active and inactive neuronal Rab2 in flies

ABSTRACT: Neuropeptide-containing dense core vesicles (DCVs) are generated in the neuronal cell body and circulate throughout the axonal arbor to supply distal release sites. This circulation depends on the anterograde kinesin-1 and kinesin-3 motors and the retrograde dynein-dynactin motor, however the adaptor proteins involved are poorly understood. Knock-out of the small GTPase Rab2 selectively disrupts retrograde DCV transport in Drosophila axons indicating that it is involved in regulation of dynein. To identify specific interactors of active neuronal Rab2 in flies, we performed proximity labeling based on a pan-neuronally expressed constitutively active GTP-locked TurboID-Rab2Q65L chimera, as well as an inactive GDP-locked TurboID-Rab2S20N variant.

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Motor Neuron, Whole Body

SUBMITTER: Michael Wierer  

LAB HEAD: Ole Kjaerulff

PROVIDER: PXD063196 | Pride | 2026-01-12

REPOSITORIES: Pride

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Publications

A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles.

Lund Viktor Karlovich VK   Chirco Antony A   Caliari Michela M   Larsen Andreas Haahr AH   Tang Kristoffer Tollestrup KT   Gether Ulrik U   Madsen Kenneth Lindegaard KL   Wierer Michael M   Kjaerulff Ole O  

The Journal of cell biology 20260106 3

Neuropeptide-containing dense core vesicles (DCVs) generated in neuronal somata are circulated in axons to supply distal release sites, depending on kinesin-1, kinesin-3, and dynein, but how the motors are recruited remains unclear. Here we use proximity proteomics in the living Drosophila nervous system to identify the protein complex responsible for recruitment of kinesin-1 and dynein on DCVs. We find that the dynein and kinesin-1 adaptor Sunday driver (Syd/dJIP3/4) interact with the DCV-locat  ...[more]

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