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LFQ proteomic profiling of Streptomyces coelicolor biosynthesis pathway mutants

ABSTRACT: Streptomyces coelicolor A3(2) is a model organism for antibiotic production at high potency. Its chromosome contains over 20 gene clusters responsible for secondary metabolite biosynthesis. This complexity of putatively competing regulatory and biosynthetic pathways can however negatively impact the yield of desired metabolite biosynthesis. Hence, a better understanding of these pathways is crucial for design of high-scale bioengineering processes. For this study proteome changes of certain S. coelicolor biosynthesis mutants were monitored. These were a double mutant with scbA protein deletion and atrA protein overexpression (∆scbA-atrAOE), as well as, an atrA protein deletion mutant (ΔatrA). These two mutants were relatively assessed against, respectively, a single mutant with scbA protein deletion (∆scbA-atrAOE vs ∆scbA-Φ) and a wild-type strain (ΔatrA vs M145).

INSTRUMENT(S):

ORGANISM(S): Streptomyces Coelicolor A3(2)

TISSUE(S): Cell Culture, Cell Line Cell

SUBMITTER: Michał Tracz

LAB HEAD: dr Michał

PROVIDER: PXD066756 | Pride | 2026-04-02

REPOSITORIES: Pride

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			Action	DRS
	145_332_50_qip_output.csv	Csv
	240214_ZCHB_BBE_23_r0_nr10.raw.zip	Raw
	240214_ZCHB_BBE_25_r1_nr10.raw.zip	Raw
	240214_ZCHB_BBE_26_r0_nr10.raw.zip	Raw
	240214_ZCHB_BBE_28_r0_nr10.raw.zip	Raw

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