Dataset Information

Liver and kidney under opioid exposure, DIA data

ABSTRACT: In the presented investigations we performed a quantitative proteomic analysis of rat liver and kidney tissues following exposure to morphine (10 mg/kg, once daily, for 10 days, intraperitoneally), compared to untreated controls. Although opioids are generally considered safe for both organs during short-term pain therapy, our data revealed significant alterations in protein expression that extend beyond classical metabolic enzymes. Notable, differences were observed in proteins related to pro- and anti-apoptotic signaling, oxidative stress response, transport mechanisms, transmembrane receptors, nucleic acid metabolism, and regulators of alternative splicing, among others. Liver tissue exhibited primarily adaptive changes, including upregulation of metabolic enzymes and subtle indications of oxidative stress based on marker profiles. In contrast, the kidneys displayed a broader spectrum of proteomic alterations, affecting a wider array of functional pathways, suggesting that this organ may be more susceptible to morphine-induced toxicity or that of its metabolites. Our results should be regarded as preliminary; however, they highlight a promising direction for identifying early-stage protein markers of toxicity using proteomic approaches. Monitoring such markers could prove valuable in multidrug therapies, particularly for patients with a history of hepatic or renal impairment, and may contribute to the development of safer therapeutic strategies.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Liver, Kidney

SUBMITTER: Piotr Suder

LAB HEAD: Piotr Suder

PROVIDER: PXD068084 | Pride | 2026-01-26

REPOSITORIES: Pride

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Publications

Liver and Kidney Tissues Under Opioid Exposure: Rewriting and Old Story Through Proteomics and MALDI-MSI.

Hopcias Malgorzata M Kret Paulina P Kotlinska Jolanta H JH Link-Lenczowski Pawel P Bodzon-Kulakowska Anna A Suder Piotr P

Journal of proteome research 20260124 2

A proteomic analysis of rat liver and kidney was performed following exposure to morphine (10 mg/kg, 10 days) versus untreated controls. Our data revealed alterations in numerous protein expression. Differences were observed in pro- and antiapoptotic signaling, oxidative stress response, transport mechanisms, nucleic acid metabolism, and regulators of alternative splicing, among others. Liver tissue exhibited primarily adaptive changes, including upregulation of metabolic enzymes and subtle mark ...[more]

PMID: 41579115

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